FastQCFastQC Report
Wed 5 Dec 2018
HGCFKBGX9_n01_CrossApool.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCFKBGX9_n01_CrossApool.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36618545
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC791210.21606811521320685TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG322450.021.05838846
GTATGCC338700.020.03750645
ATGCCGT340450.019.80026247
CGTATGC344400.019.65481844
CTGCTTG315600.019.47321757
TGCTTGA335150.018.7653558
TCGTATG367000.018.20606643
TGCCGTC372800.017.86673248
GCCGTCT382750.017.19191649
CTCGTAT376550.016.721842
GCTTGAA395450.016.45277459
CCGTCTT421400.015.86413250
TCTGCTT425450.014.70032156
TCTCGTA435500.014.474442541
ATCTCGT466250.013.24200240
GATCTCG469200.013.15101739
CTTCTGC505100.012.65259354
AGATCTC507100.012.31301738
TTCTGCT526650.011.92852155
GAGATCT540550.011.57000437
Produced by FastQC (version 0.11.5)