FastQCFastQC Report
Wed 5 Dec 2018
HGCFKBGX9_n01_C.F1.fcWGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGCFKBGX9_n01_C.F1.fcWGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31888782
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC404410.12681889198527557TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA169300.017.79969460
GCTTGAA175700.017.01143359
TGCTTGA208500.013.93242858
CTGCTTG219250.013.00986657
GTCTTCT228300.012.95492452
GCCGTCT298200.010.98644249
TCTGCTT275250.010.79524456
TCGTATG322100.010.70337543
GTATGCC344650.010.40952845
CGTATGC353000.010.14325844
CTCGTAT340450.010.00308742
CCGTCTT348750.09.65488250
TCTCGTA368350.09.21694541
TTGAAAA367050.09.17324561
CGTCTTC359700.09.04959951
TTCTGCT327400.09.03281955
AAAAGGG130650.09.02804470
TATGCCG407100.08.95891746
CTTCTGC344350.08.93407854
ATGCCGT411100.08.67552647
Produced by FastQC (version 0.11.5)