FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40623236
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC436082510.73480458326855No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC418575510.30384433184988No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT30571507.525619081650708No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA30006377.386504117988039No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA28304326.967519771196957No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG24326725.988375716794201No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC21148445.205995898504984No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA13606633.349469746821745No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC8153452.007090227868602No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC7606911.872551462911522No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC7060281.7379905431462919No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC6485861.5965887109535046No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC6276621.5450812436508012No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT4893741.2046652314946056No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGTACCCCTACCA4880401.20138139659775No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA4754801.1704631310021685No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA4396131.0821712972349125No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG4121131.0144760501108283No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC3612690.889316153937121No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTACCCCTACC3582540.8818942932069714No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA3351460.825010592459941No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3088920.7603825554419151No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2911430.716690812125356No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2827500.6960302227030855No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT2732830.6727258261749507No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC2640620.6500269944029078No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC2406370.5923629520799377No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA2148310.528837732178697No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA1934490.47620283130570884No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG1763020.43399299848982986No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1666960.41034643325804965No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTTCCCCTACC1288980.31730116232000816No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1146970.282343336705131No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC1146740.2822867188620818No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAA1084100.2668669723898904No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC1082810.26654942013974464No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA992550.24433060921094518No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT965450.23765955031253544No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTTCCCCTACC905470.22289460150343512No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACT870320.21424191809830215No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA693920.17081849412488953No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACT682600.1680319115887272No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC607340.1495055686848778No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCGCTACCAAC605920.1491560150451825No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA600300.14777257035850122No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTG591600.1456309389040302No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA559460.1377192107492372No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTACCCCTACC545020.1341645948638853No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC517760.12745414963987606No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA502430.1236804473183771No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAA493120.1213886554975581No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG487300.11995597790387746No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT429370.10569566639152037No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAACT415620.10231090403531615No Hit

[OK]Adapter Content

Adapter graph