FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37194098
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC411062311.05181526380879No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC36142409.717240622423482No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT27712287.450719735157982No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA27011547.2623188765056215No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA27010167.261947849898121No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG20441465.495888084179377No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC19648945.2828112675295955No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA10896362.9295938296446926No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC7905242.1254017236820744No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC7633932.0524573549276557No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC6081601.6350981276653087No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC6020361.6186331498078002No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC5740791.5434679986055853No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA4820031.295912593444261No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGTACCCCTACCA4696201.2626196769175582No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT4547621.222672478843283No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA3943721.0603080090825163No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC3613550.9715385489385978No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG3557090.9563587212143174No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTACCCCTACC3341340.8983522063097216No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA3213500.8639811617423818No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC2903150.7805405040337314No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT2790010.7501216994158589No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC2571060.6912548329576375No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2521040.6778064627350285No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2430810.6535472375213939No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC2180260.5861843994711204No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA2012710.541136929843009No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA1731610.46556042305421685No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG1660760.4465117019372267No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1553880.41777596004613415No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTTCCCCTACC1297480.3488402918118891No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAA1089060.29280451968481663No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC1016570.27331486839659347No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG982730.26421665071700356No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC975950.2623937808627595No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACT904810.24326709038622205No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT893440.24021015377224636No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTTCCCCTACC880940.23684940551589664No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA870280.23398335940288162No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA661930.17796640746604475No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACGGAACCCCTACCAACTG644520.17328555729460088No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACT640860.1723015302051417No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCGCTACCAAC626140.16834391305846427No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA586470.15767824239211287No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC558320.1501098373188133No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA542150.14576237337439935No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTACCCCTACC531690.14295009923348592No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA512240.13772077494660576No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC490470.13186769578334714No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAA464490.1248827166073499No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCGTACCAACT429280.11541616091886406No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG422060.11347499272599648No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGTACCCCTACCAA392700.10558126722148228No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATGACCGAACCCCTACCA391460.1052478809944524No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACT390820.10507581068372729No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT384020.10324756363227305No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC377030.1013682332073223No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC373750.10048637286485614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)