FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29392084
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA302673710.29779650874705No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC25291068.604718195552245No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA24667438.392542019136853No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC21911537.454908607365167No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC19228826.542176458123896No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT16910565.753440280042748No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG14970685.093439444443613No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA14838475.048457945343379No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC6826112.3224314410641997No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC6550912.228800788674937No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC5571181.8954695420712597No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA4455801.5159864132124827No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGTACCCCTACCA4216781.4346651976089888No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC4122311.4025238904461488No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC3865451.3151330133650951No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA3375531.1484486775418852No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTACCCCTACC3195311.087132848422725No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2825380.961272429678685No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC2781190.9462377693259179No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT2742240.9329859019183533No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2689460.9150286859550346No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG2498340.8500043753277243No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCA2273390.7734701629186961No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC2266350.7710749601831568No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1784380.6070954342672673No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC1621610.5517165778377606No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1601120.5447453130577607No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1375160.4678674707108213No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA1331230.45292126954999173No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT1135960.38648501412829384No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTTCCCCTACC1112970.3786631801950484No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC1066770.362944662243072No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA980040.33343671717867984No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTG891700.3033810055795976No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGTTCCCCTACC798770.2717636490151566No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGTACCCCTACCAA785770.2673406894182801No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG700820.2384383495909987No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA616620.20979118050969098No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA596070.20279950207001313No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC534840.18196736236872485No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT526370.17908563407752917No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGTACCCCTACC462130.15722940911573333No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCCCTACC455300.15490565418906668No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAA407480.13863596742578715No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA407350.1385917378298184No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA385640.13120539530303466No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC364780.12410824628835437No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGTACCCCTACCAACT363010.12350604332785657No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA360040.12249556717380095No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC359030.12215193723588977No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACT337660.11488127211394743No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC306770.10437163965644627No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC300780.10233367596527011No Hit
GATCGCGAGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTG294890.10032973504022376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)