FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12694689
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA230831018.183273335802085No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA177534613.984950714428686No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC12093849.526692619252035No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC10963308.636131219914093No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC9875377.779135038282544No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA5047903.9763872907796323No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC4281083.3723394090237266No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC4199153.3078006085852123No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC3551712.797792053038873No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA3042452.396632166412269No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2361431.8601716040463852No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2159741.70129413962012No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1761641.38769843042236No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1480571.1662908795953961No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1245200.9808826352500639No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA1225940.965710936282094No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC745800.5874897762363458No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC611850.48197320942639876No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA505400.39811924498504847No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA458560.3612219251688639No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATGACCGAACCCCTACCA428830.33780268268092273No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA419620.3305476802149308No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA353100.27814781441278313No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA324930.2559574322773878No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC242240.19081995628250523No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC229600.1808630365029029No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA226740.17861012585656885No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC223690.17620754632114263No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC199670.15728624781591735No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCAA176540.13906602989643937No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC147800.11642664109376764No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCTCCGAACCCCTACCA144560.11387439266924931No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC137420.10824999336336637No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA127890.10074291697890354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)