FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10158222
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA156131615.369973209878657No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA142539114.03189455792559No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC9476819.32920150790168No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC8185758.058250745061487No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA7905587.782444604971224No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC7781497.660287400688821No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3407703.354622492006967No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC3125963.0772708058555915No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA3027732.980570812490611No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC2661922.620458580251544No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1929371.8993186012276557No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1375701.3542724307462468No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA1342241.3213335955839516No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1328281.3075910331552116No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1122421.104937458543434No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA842090.8289738105743307No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC603330.5939326783761961No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA568210.559359698970942No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC465310.45806244439233557No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA414470.40801431589110776No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA387260.3812281322459777No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA316070.3111469704048602No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA286990.28251991342579436No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC190990.1880151861221383No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA184560.18168533824127883No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC181680.17885019642216915No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC176710.17395760793571946No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATGACCGAACCCCTACCA172650.16996084551016902No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCAA166720.16412320975068276No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC156220.15378675520184537No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA133030.13095795701255594No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA132660.13059372004273975No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC116840.11502012852249144No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC104870.10323657033681681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)