FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9646066
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA160252716.613270114469465No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA149947915.544979683945767No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC8778009.100082873163009No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC7386617.65763991247831No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC7360187.630240141421384No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA6508626.747434653671249No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC3075503.188346420188292No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC2966603.0754506552204806No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC2571032.6653663783764285No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA2371522.4585359461567027No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1740181.804030783119253No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1491121.5458322594931446No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1273161.3198748588284592No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1078201.1177613754664337No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA969131.0046893728489936No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA879580.9118535991771154No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC580960.602276617224058No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA500220.518574100571155No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC425950.44157898152469616No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA367280.3807562585617805No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA319610.33133714822187615No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACGCCTACCAA275960.2860855399496541No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA254390.2637240922879856No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATGACCGAACCCCTACCA188510.19542681959671435No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC181100.1877449314570313No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC171160.17744021241405564No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA168000.1741642655150815No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC158290.16409798564513242No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC139000.14410019587259718No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCAA132110.1369573876023656No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC104840.10868679521786394No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA101580.10530717911322607No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC99040.10267398128936708No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA97460.10103600783988001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)