FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31625771
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT688797521.779627127509396No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC525734416.623607373872403No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA18671285.903818123517052No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA15463464.889512416946293No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC11718233.7052788373127727No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC9781203.092794164607086No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA9112152.881242009878589No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC8919562.820345470787099No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT8271782.6155188437935633No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC7484402.3665510004483368No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC6642912.100473692799458No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGAACCCCTAC5084441.6076888686761186No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT4074721.2884176009495547No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3910641.2365358618450757No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3238631.0240477615549672No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC2985400.9439769863634312No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC2633940.8328460988350291No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC2535470.8017100990201945No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT2443940.7727685121099498No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA2266570.7166845039129639No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1812510.5731117195530189No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1753800.5545477452549694No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1417250.44813136729536174No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1288240.4073386859090328No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC1148660.36320379351384036No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1102460.3485954540049No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA997710.31547373184988914No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA904120.2858807774204145No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT894580.2828642501711658No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTGGT848170.26818950911900297No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGATCCCCTAC817940.258630848873218No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT812120.25679057753248136No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGATCCCCTAC651930.20613884796674206No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA609480.19271625030105985No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGATCCCCTAC570280.18032129556620138No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGTACCCCTAC567950.179584554634257No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC541060.17108199512353392No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTGGT534970.16915635037008267No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC488650.15451006712215806No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTGGGCACTCAGTCTC436460.13800770264225337No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA387290.12246025559345255No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA370340.11710070246192575No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT370010.11699635717971903No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGTACCCCTAC318750.10078805667694235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)