FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35075529
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT773190022.0435734554424No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC556603815.868721466752506No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA18167485.17953129088944No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA17350644.946650982797722No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC13961233.9803334113649433No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT10953103.1227184057580426No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC10213492.9118562973063074No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC9200082.6229340689344984No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC8817582.5138836822674864No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC8229642.3462625467459093No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA8106242.3110813239623558No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGAACCCCTAC7356372.097294099256493No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT5821821.6597953519104445No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3769431.0746609124555184No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC3736031.06513860418185No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT3299100.9405702762173593No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3292460.93867721852463No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC3052450.8702505955077685No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA2901280.8271521721026645No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC2834240.8080391317833011No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2047980.5838771526439416No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT2027680.5780896419267119No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1698820.4843319683075913No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1490780.42501996192274105No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTGGT1374980.39200549192002204No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC1358960.3874382051372625No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1285010.36635513038164014No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGATCCCCTAC1211540.3454089031700705No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA1190020.33927357161170685No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT1086310.30970594912481575No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1014500.28923298633642847No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT991270.28261013540237695No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTGGT870140.24807608746257254No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGTACCCCTAC858980.24489438206334677No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGATCCCCTAC826900.23574840453582324No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGATCCCCTAC825500.23534926586566948No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC736200.20988992069086113No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA602850.17187196235871452No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC479320.1366536766986465No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGTACCCCTAC427900.12199388354199876No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA424430.12100458983811761No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAGCAACTGGT410660.11707877591810517No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT402080.1146326260681628No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGGT384470.10961203179572858No Hit
ATCGATTAATTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA365470.10419514984364171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)