FastQCFastQC Report
Tue 26 Jul 2022
HG7LFBGXM_n01__R13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7LFBGXM_n01__R13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34557955
Sequences flagged as poor quality0
Sequence length130
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT849892524.59325211807238No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC625106318.08863689995545No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC15060074.357917012161165No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA14834074.292519623918718No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA13596523.9344110494964184No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT11210293.243910121417775No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGAACCCCTAC9442302.732308668148911No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC8195042.3713903209839815No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGAACCCCTAC7417672.1464435612581823No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC6728561.9470365072238796No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC6623131.9165283362398038No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA6469361.8720320690272327No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT5812591.6819832076290393No Hit
TAACAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT4102581.1871593675030827No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT3407310.9859698005857117No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC3137270.9078286027052237No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC2815420.8146951982546421No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC2803330.8111967273526458No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGATCCCCTACC2345170.6786194379846839No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA2165110.6265156604318746No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACTGGT1963310.5681210013729111No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1589420.45992883548809527No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAC1458290.42198388185875No Hit
ATCGATTACTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1377940.3987330847557386No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTAGCAACTGGT1300400.37629541447113984No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGATCCCCTAC1194650.3456946454152163No Hit
CGATCGTTACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1184710.3428183178084467No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACTGGT1125980.3258236779346463No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGGT1105770.31997553095951425No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGAACCCCTACC1002370.2900547789937223No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGTCCGAACCCCTACCAA875330.2532933444701806No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGATCCCCTAC863090.2497514682220056No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTTACACCGTACCCCTAC851620.2464324060842142No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGTAACACCGATCCCCTAC820700.23748511739192898No Hit
ATCGATACCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA815210.2358964817217917No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTTCCCCTACCAACTGGT811570.23484317865452398No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAAC525250.15199105386878362No Hit
GATCGAAAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA477860.1382778581660865No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGTACCCCTAC439750.12725000654697305No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT438380.12685357105187503No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTACCCCTAGCAACTGGT394040.11402295072147643No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC387100.11201473003827916No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACTGGT383630.11101061969668055No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTGGT381370.11035664581425608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)