FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22262573
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1187080.5332177911331273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG432210.19414197990501816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT948350.035.703172
CTTTGTG950650.035.1574551
TGTGTTT1035950.034.424674
TTGTGTT1070800.033.1688043
ACTTTGT537950.032.739241
CTTGTGT55650.027.4734152
CCTTGTG35550.026.6173691
GTGTTTG1386200.025.7064725
GATTTTT182150.020.1591286
GGTAACT62550.019.8727551
TGATTTT188700.019.7738385
CAGTCAC46600.019.23145128
TTGAGGG154950.019.2160479
TGTTTGA1869150.019.092466
GTTTGAC502400.018.5498757
TTCCGAT33400.017.034385
TTTGAGT281300.016.5783183
CCAGTCA54800.016.5436127
GTTTGAG997200.016.3425927
TTGATCG66300.016.21525
Produced by FastQC (version 0.11.5)