FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22892143
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371570.1623133316963816No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAGAACATCTCGTA268980.11749882918344516TruSeq Adapter, Index 15 (97% over 36bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258110.11275047513026631No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC230860.10084682766484553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35800.056.3184446
TATGCCG36850.055.9567649
CGTATGC37500.054.70115747
CTCGTAT37650.053.54996545
GTATGCC39650.051.913448
ATGCCGT45100.045.8764250
GTCACAC47300.044.83217230
CCGTCTT47250.043.04883653
AGTCACA50400.042.21359329
CAGTCAC52800.040.29382328
CATCTCG50700.039.9003742
GCTTGAA53900.037.74668562
TTTGTGT1555350.035.2326432
TGCCGTC58600.035.00905651
ACTTTGT882100.033.5703741
CCAGTCA64950.032.80933827
TCCAGTC65050.032.7051826
TGCTTGA64100.031.73950661
TGTGTTT1827800.031.6267384
CTTTGTG1757250.031.1438451
Produced by FastQC (version 0.11.5)