FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23621004
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTA395860.16758813469571401TruSeq Adapter, Index 18 (97% over 37bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349950.14815204298682647No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC268880.11383089389426461No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263760.11166333149937233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52150.060.81242846
CGTATGC53250.059.6912247
TATGCCG53950.058.98686249
CTCGTAT51950.058.6202345
GTATGCC54100.058.43132448
CTATCTC55000.054.9785341
ACGTACA61500.052.51909633
TATCTCG57300.052.4676642
ATGCCGT61800.051.7785350
GTCACGT62800.051.3743430
CCGTCTT60650.051.14544353
ACCTATC60050.050.52794639
CCTATCT60000.050.3962440
CACCTAT63300.050.0316338
TCACGTA65100.049.9370131
AGTCACG67650.047.8463629
CGTACAC67650.047.7445334
GTACACC68850.047.1166835
GCTTGAA66350.046.92046762
CAGTCAC72500.044.7894528
Produced by FastQC (version 0.11.5)