FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14955162
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC259520.1735320553531951No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCATTATCTCGTA192010.1283904514040035TruSeq Adapter, Index 22 (97% over 38bp)
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC179000.119691114011336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27850.049.27418549
TCGTATG28550.048.55019846
GTATGCC29550.047.02928548
CGTATGC30550.045.71765547
CTCGTAT30000.045.03579345
TTATCTC31400.043.6908141
ATTATCT32150.042.77844240
TTTGTGT1370350.042.566642
CCGTCTT32650.041.60591553
CATTATC34650.040.60067439
CTTTGTG1442500.040.2664261
TGTGTTT1555000.039.221634
ATGCCGT35100.038.9980450
CCTTGTG39800.038.692351
GCTTGAA35950.038.4804362
ACTTTGT574500.037.941461
TATCTCG36150.037.75748442
TTGTGTT1644650.037.0030633
CCATTAT38800.036.8867238
TCCATTA39850.036.35446537
Produced by FastQC (version 0.11.5)