FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15141410
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCATCCTATCTCGTA248650.1642185239023314TruSeq Adapter, Index 1 (97% over 37bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193600.12786127579928158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC33400.056.48996447
TCGTATG33650.055.23605746
TATGCCG35100.053.65815449
CTCGTAT34200.053.3233845
GTATGCC36000.052.60547348
CTATCTC37250.050.82826241
TATCTCG38300.049.1624642
CATCCTA43650.048.8249237
GTCACAC47200.045.29994230
ATGCCGT41900.044.8672550
CCGTCTT41050.044.5182453
AGTCACA49800.043.07599629
CCTATCT45400.042.47411340
CAGTCAC52450.040.76495728
ACTTTGT514750.039.5920261
TCACACC54350.039.27665331
TGCCGTC47750.039.0776851
GCTTGAA48650.036.27666562
TTTGTGT928750.036.1289332
CCAGTCA62100.034.42928327
Produced by FastQC (version 0.11.5)