FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14079729
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235110.166984748072921No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCCTTCATCTCGTA183750.1305067732482635TruSeq Adapter, Index 27 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26550.057.3491746
TATGCCG27600.055.0503749
CGTATGC27550.055.01616747
CTCGTAT27900.052.06413345
GTATGCC29700.050.91818648
CAGTCAC37500.042.5491628
ATGCCGT35850.042.38232450
CCGTCTT35500.041.5187453
ACTTTGT516850.040.480941
AGTCACC39750.040.05354329
CATCTCG35500.039.04007342
GTCACCT41400.038.2036430
TTTGTGT964950.037.09322
CCAGTCA43400.036.8454527
TGCCGTC41300.036.1116151
TCCAGTC44600.035.46143726
TGTGTTT1105450.034.248944
GCTTGAA43700.034.13903862
ACTCCAG46800.034.0927924
CTTTGTG1092350.032.7196161
Produced by FastQC (version 0.11.5)