FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8361924
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101090.12089322983562156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT252150.039.1854061
TTTGTGT504450.038.238612
TGTGTTT575100.035.3599664
CTTTGTG559050.034.4525451
TTGTGTT602100.033.6987723
GTGTTTG760750.026.7353175
CTTGTGT37200.025.771512
CCTTGTG25650.024.1522921
TTGAGTC50550.024.0180594
TTTGAGT117050.023.0767963
GATTTTT94250.022.75646
TGATTTT99600.022.0611085
TTGATTT134850.020.0566394
GTTTGAC264450.019.9121117
TTTGACG98400.019.272088
TGTTTGA1067600.019.0542056
TTGACGC29350.018.8353379
TTGAGGG83500.017.9341879
TTGAGGT46000.017.798469
TTCCGAT18500.017.5888825
Produced by FastQC (version 0.11.5)