FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8134595
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97030.11928067715725245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT446850.037.805862
ACTTTGT235750.036.4063571
TGTGTTT513300.034.8065074
CTTTGTG514200.032.8453331
TTGTGTT544650.032.687413
CTTGTGT32400.026.6750682
GTGTTTG696900.025.626455
CCTTGTG22450.025.5703071
GATTTTT92450.023.162896
TGATTTT98800.022.0991675
TTGATTT129000.020.4247234
TTGAGTC43500.020.1899574
TTTGAGT106550.019.9336193
TTGACTT38400.019.0443424
GTTTGAC239150.018.859417
TGTTTGA970150.018.408576
TTTGACT95050.018.3327733
TTGAGGG77150.018.27739
TTCCGAT17300.017.5963025
TTGAGCG43950.017.5944759
Produced by FastQC (version 0.11.5)