FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8164775
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117450.14384964680594384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT230850.039.524621
TTTGTGT451800.035.9725342
CTTTGTG481350.033.6939241
TGTGTTT518400.033.092214
TTGTGTT553100.030.9973073
GTGTTTG701850.024.4274435
CTTGTGT36650.022.5304362
TTGAGTC48450.021.6648834
GATTTTT107450.021.4589216
TGATTTT111850.021.083865
TTTGAGT113250.020.9470583
CCTTGTG25100.020.218591
TTGATTT141550.019.626354
TAACTTT60650.018.8123651
GTTTGAC246000.018.561017
TGTTTGA987300.018.4832551
GGTAACT34150.017.5251581
TTTGACT106200.016.7696823
TTTGACG97050.016.3676598
TTGACGC29100.016.3520479
Produced by FastQC (version 0.11.5)