FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9104626
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103190.11333798884215562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT481150.037.572622
ACTTTGT246400.035.3415371
TGTGTTT564200.033.796724
CTTTGTG547800.032.8665921
TTGTGTT597000.031.8402523
GTGTTTG784550.024.250875
CTTGTGT36700.024.0241492
CCTTGTG23400.022.585871
TTGAGTC51250.020.4804064
TTTGAGT120350.019.7103713
GATTTTT104900.018.9776826
GGTAACT48000.018.7399161
TGATTTT110550.018.3559975
GTTTGAC267500.018.2847827
TAGTATC11600.018.10068739
TGTTTGA1079650.017.6871136
TTTGACG104300.017.2418088
TTGATTT143700.017.238034
TTGACGC32850.016.7212319
TTTGACT108800.016.2395363
Produced by FastQC (version 0.11.5)