FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353031
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100040.1197649092886163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT244850.038.0510031
TTTGTGT477300.037.694612
TGTGTTT550550.034.497034
CTTTGTG531850.033.712691
TTGTGTT578200.032.8291973
CTTGTGT33550.027.0113222
CCTTGTG23250.026.7950921
GTGTTTG742000.025.5631335
TTGAGTC48700.022.4163154
TTTGAGT121300.021.518733
GATTTTT88100.021.2479236
TGATTTT91850.020.7232745
TTCCGAT19450.020.328135
TTGATTT124600.018.9831054
CGTATGC4250.018.94392447
TGTTTGA1049150.018.9115471
TTGACTT41150.018.7914814
GTTTGAC262600.018.720627
TTTGACT109450.018.2220693
TTGATCG39500.017.4504955
Produced by FastQC (version 0.11.5)