FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8461367
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108320.12801713954730956No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC88980.10516031274851925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT284550.042.536191
TTTGTGT608600.039.2844052
CTTTGTG643900.037.0186731
TGTGTTT698650.036.033314
TTGTGTT745700.033.6993873
CTTGTGT39250.028.2592322
CCTTGTG25100.028.1688941
GTGTTTG896450.028.078565
TTGAGTC70800.024.610994
TTTGAGT153900.024.1904373
GATTTTT83600.022.098266
TTGAGGG109400.021.4602959
TGATTTT88600.021.4435425
TGAGGGG50300.021.42462310
TTCCGAT20600.020.721595
TTTGACG119600.020.4784628
GTTTGAC328700.019.84167
TGAGTCT17050.019.7005025
TGTTTGA1279200.019.6853686
TAACTTT72550.019.3945981
Produced by FastQC (version 0.11.5)