FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166650
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT385500.038.110192
ACTTTGT202800.037.4310871
TGTGTTT444000.034.7511524
TTGTGTT471150.032.7119453
CTTTGTG447450.032.7099881
GTGTTTG595350.025.9106485
CTTGTGT26500.025.2177942
TTTGAGT97750.023.2657053
TTGAGTC40950.022.8123974
GATTTTT56150.022.680846
CCTTGTG18450.022.1937331
TGATTTT60050.022.0236515
TTGACTT37100.020.1815014
TTCCGAT16150.020.147495
TTGAGGG75700.019.1804929
GTTTGAC210700.018.6974077
TGTTTGA841150.018.6815851
TTGATTT88200.018.4854874
TTTGACT91800.018.4468543
TTTGACG80700.017.6019178
Produced by FastQC (version 0.11.5)