FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056839
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98200.13915578915715662No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.1071301187401328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT220200.039.514651
TTTGTGT443100.037.934742
TGTGTTT511400.034.7357064
CTTTGTG490150.034.2471541
TTGTGTT536400.033.0454943
CTTGTGT32000.026.5700342
GTGTTTG683800.025.9883565
CCTTGTG19350.025.5042441
TTGAGTC46100.021.7067494
TTTGAGT109000.021.2825133
GATTTTT81550.021.152066
TTCCGAT14750.020.8747045
TGATTTT85100.020.8452955
TGAGTCT12550.019.5156945
TTGATTT113500.019.3902974
GTTTGAC240300.019.365457
TGTTTGA948000.018.7419196
TTTGACG89250.018.7389958
TAACTTT61700.018.379521
TTGAGGG80200.018.192339
Produced by FastQC (version 0.11.5)