FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9563418
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.1562621230191967No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC113450.11862913447890702No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107950.11287805259583969No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102700.10738838352563906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT661150.037.3267562
ACTTTGT358800.035.2157861
CTTTGTG737700.033.3405531
TGTGTTT775700.033.3118324
TTGTGTT840850.030.6229213
CTTGTGT37600.026.2426452
TTCCGAT16650.025.427915
GTGTTTG1016200.025.4210365
CCTTGTG25850.024.372191
CCGATCT21150.020.017617
TTGAGTC64900.020.0018884
GATTTTT94700.019.2497466
TGTTTGA1344100.019.2167426
GTTTGAC385550.018.9308827
TGATTTT96550.018.9172385
TTTGAGT146200.018.835353
TTGAGGG117300.018.8221239
TTGACTT46950.018.7059564
TTGATCG46400.018.2489095
TGAGTCC23050.018.2157710
Produced by FastQC (version 0.11.5)