FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6309363
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147810.23427087647358377No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133220.211146513522839No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCTAACATCTCGTA117850.18678589264875076TruSeq Adapter, Index 13 (97% over 37bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101150.16031729352075635No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC88230.13983979048281103No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85350.13527514584277367No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC74700.11839547035096887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13400.058.7771647
TCGTATG13550.058.12511446
TATGCCG13900.056.91922449
CTCGTAT13550.056.0571145
GTATGCC14700.054.05887248
GTCTAAC14900.053.54866436
CGTCTAA15350.052.20681435
CTAACAT16450.047.43787838
ATGCCGT17050.046.198450
CCGTCTT17100.045.24584253
CATCTCG16800.044.79069542
CCGTCTA18050.044.58959234
GCTTGAA18850.041.6140362
ACCGTCT19950.040.1675633
TGCCGTC19850.039.68174751
TCTCGTA18900.039.63096644
CAGTCAC20500.039.08896628
AGTCACC21250.037.87432529
TGCTTGA20700.036.87898361
TCACCGT21850.036.67385531