FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7721917
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.12766259984405426No Hit
CTTTGTGTTTGAGAGGTGTAGGATAAGTGGGAGCCTCCGGGCGCAAGTGA80810.10465017948263365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT300650.041.907241
TTTGTGT603700.037.276722
CTTTGTG658300.034.0626681
TGTGTTT705600.033.524294
CGCGTAT2500.032.20350345
CTTGTGT32250.032.112552
TTGTGTT738300.031.9830113
TATGCCG4900.031.43871349
CCTTGTG22200.030.584161
CGTATGC4800.029.1717347
AATCCAG18600.028.97207635
CACAATC18500.028.93841232
TCGTATG3400.027.79750346
CTCGTAT4350.027.359345
ATCGCGT4400.027.04524443
GTATGCC6000.026.83920948
GTCACAA20800.025.73832330
CCAGCAT20600.024.97035838
GTGTTTG980550.024.0986565
TTGAGTC63200.023.140164
Produced by FastQC (version 0.11.5)