FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11428173
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGAGGTGTAGGATAAGTGGGAGCCTCCGGGCGCAAGTGA147360.12894449532746835No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC130760.11441898893200164No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTACATATCTCGTA117960.10321859845838877TruSeq Adapter, Index 11 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16550.057.11560449
TCGTATG16750.056.84269746
GTATGCC16850.056.30303648
CTCGTAT17550.052.85493545
CGTATGC18300.052.41346747
ATGCCGT19750.048.21638550
ACATATC19600.047.67307339
CTACATA20550.046.8295337
CCGTCTT20050.046.62269253
TATCTCG20350.046.09208342
CATATCT20900.044.70854640
GCTACAT22100.043.3865736
ACTTTGT507050.043.1371041
GCTTGAA22300.042.55684762
TGCCGTC24100.040.0946851
TACATAT25000.038.913338
TGCTTGA24600.038.29224461
TTTGTGT1004500.037.767042
TGTGTTT1154800.034.269194
CTTTGTG1115400.033.9640121
Produced by FastQC (version 0.11.5)