FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_16b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_16b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17151849
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCCAAATCTCGTA172410.1005197748650889TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC29200.049.98940747
TCGTATG28850.049.9862846
CTCGTAT31100.045.4693945
TATGCCG32000.045.18185849
GTATGCC34050.042.87078548
ACTTTGT508900.038.9119571
ATGCCGT41850.034.12980350
CAGTCAC45600.033.84116428
CCGTCTT42600.033.03710653
TTTGTGT890050.032.384022
CAAATCT45050.031.46189940
AGTCACC49650.031.0108629
TGTGTTT1000500.030.4525384
CTTTGTG942450.030.4031811
TTGTGTT1033650.029.3507423
GCTTGAA51050.028.19410562
TCTCGTA51250.027.79591444
CCAGTCA58050.026.5226527
TCCAGTC58700.026.34794826
ACTCCAG59600.025.94962324
Produced by FastQC (version 0.11.5)