FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_16a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_16a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7704799
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78780.10224796260097117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10400.043.0832247
TCGTATG9800.042.8611846
TATGCCG11950.039.5486149
GTATGCC11800.039.4564648
TCACTCC18650.039.4018831
AGTCACT18800.039.08674229
CTCGTAT11300.037.7906845
TTTGTGT446600.037.7318042
ACTTTGT216900.035.7592281
CTTTGTG470350.035.6965751
CAGTCAC20950.035.5760728
TGTGTTT501400.035.380464
TTGTGTT514450.034.312933
ACTCCAT24100.031.07248733
CACTCCA24100.030.78189332
CCAGTCA23950.030.68166727
ATTGCAT23300.030.18872638
CCATTGC25300.029.60003936
ACTCCAG25550.028.62298624
TTGCATC23950.028.20212439
Produced by FastQC (version 0.11.5)