FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_15b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_15b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470896
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122780.10703610249800886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11450.043.41293347
TCGTATG11200.041.87993646
TTTGTGT787500.038.474972
TATGCCG13250.038.31141349
ACTTTGT385600.037.9209021
GTATGCC13950.036.8889648
TGTGTTT873850.036.3703424
CTTTGTG832750.036.1435741
TTGTGTT904850.034.973813
CTCGTAT13100.034.7358645
TATCTCG14000.032.99856642
GCCTTAT16900.032.30290638
TTATCTC17050.028.73700341
GTGTTTG1160450.027.3637245
CTTGTGT48050.026.6500872
ATGCCGT19500.025.673550
CGCCTTA21800.025.36310437
TTGAGTC81900.024.990744
CCGTCTT19550.024.89207853
CCTTGTG33950.023.9163841
Produced by FastQC (version 0.11.5)