FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_15a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_15a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19388828
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207330.10693271403511342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT658200.039.4074631
TTTGTGT1314450.037.0472222
CTTTGTG1374100.035.2489281
TGTGTTT1476400.034.722614
TTGTGTT1527300.033.474053
CTTGTGT84700.026.5200612
GTGTTTG1988450.025.7721235
CGTATGC14450.025.6784847
CCTTGTG59000.024.8554481
TATGCCG14300.024.48136149
TCGTATG14200.023.91069646
GGTAACT93550.022.6345121
TTGAGTC141250.021.7483144
TTTGAGT335250.021.4678443
GTATGCC17350.020.78192948
CTCGTAT15950.020.4090245
TGAGTCC51100.020.4038245
GATTTTT179600.019.9289476
TTGAGGG231650.019.7404089
TGAGGGG108900.019.11621510
Produced by FastQC (version 0.11.5)