FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_14b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_14b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8912558
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10750.049.16650846
CGTATGC11350.048.7288947
TATGCCG12900.042.8784449
CTCGTAT12350.041.37895645
GTATGCC15000.038.0398448
CTTAGGT16300.037.99962636
TATCTCG13650.037.1777142
ACTTTGT250800.036.072631
TAGGTAT16700.035.83158538
AGGTATC16950.033.65450739
TTTGTGT414400.031.65312
ACCCTTA20000.030.44444733
CCGTCTT17800.029.69959353
CTTTGTG440400.029.6896061
TGTGTTT470400.029.178754
ATGCCGT19500.028.90481450
TTGTGTT499050.027.5600223
TTAGGTA23400.026.17102437
GCTTGAA21000.023.84691662
GTGTTTG623550.021.989725
Produced by FastQC (version 0.11.5)