FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_14a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_14a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12790595
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAACATCTCGTA131230.10259882358873845TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG19300.049.6930946
TATGCCG21700.048.8840549
CGTATGC21200.048.3795347
CTCGTAT20850.044.82354745
GTATGCC25050.042.48209848
ACTTTGT401400.038.6365431
TCACAAC37350.036.16377631
GTCACAA37750.035.50166330
AGTCACA38500.035.08328229
CGAACAT39150.033.3387338
TTTGTGT707200.033.310462
ATGCCGT32250.032.4590650
CAGTCAC41800.032.14549628
CACAACC43300.031.67915332
CCGTCTT29500.031.09476953
CTTTGTG761600.030.8230251
CATCTCG31250.030.79800642
TGTGTTT808400.030.6767944
TTGTGTT819750.030.1283953
CCGAACA47350.028.60013237
Produced by FastQC (version 0.11.5)