FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_13b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_13b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15901876
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195760.1231049720171381No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGACCATCTCGTA184810.1162189920233311TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25450.057.2133946
CGTATGC26650.057.003847
TATGCCG27600.055.1737449
CTCGTAT26150.053.6730945
GTATGCC29000.053.11157248
CTAAGAC36400.049.5109235
ACCTAAG38400.047.38668433
AGACCAT39600.042.1518838
GACCATC37450.041.5833739
ATGCCGT36850.040.75468450
CCGTCTT34350.039.84998753
CATCTCG36300.038.5652642
TAAGACC47850.037.8827636
TCACCTA48550.037.1918231
GCTTGAA39500.036.6155162
AGTCACC51550.035.23050329
CAGTCAC51900.034.92505328
ACTTTGT546650.034.817371
GTCACCT53050.034.03646530
TTTGTGT955200.033.662842
Produced by FastQC (version 0.11.5)