FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_13a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_13a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11257083
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGACTTATCTCGTA194260.1725669074306372TruSeq Adapter, Index 25 (97% over 38bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140100.12445497648014145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTA27150.057.87218537
TCGTATG26950.057.53944446
CGTATGC27150.057.2485847
GACTTAT26800.057.0618438
TATGCCG27450.056.6259349
CTCGTAT26850.055.40585745
ACGGACT29550.053.2903235
GTATGCC29250.053.01944748
TTATCTC28300.052.06419841
TATCTCG28250.051.53874642
GTCACAC33000.047.61005830
AGTCACA33550.047.1435129
CTTATCT31800.046.3324240
CCGTCTT33700.045.606553
ATGCCGT34450.045.1203450
CAGTCAC35600.044.03482428
GCTTGAA37400.041.2928662
ACTTTGT388350.041.0962681
CCAGTCA39600.039.4099627
ACACGGA40500.039.139533
Produced by FastQC (version 0.11.5)