FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_12b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_12b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16215699
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226490.13967328821286087No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC162680.10032253312052722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT584700.043.0407261
TCGTATG15400.037.50296846
CGTATGC15250.037.41533347
TTTGTGT1169150.036.285162
TATGCCG16300.035.43771449
CTTTGTG1214000.034.797491
TGTGTTT1314500.033.989424
TTGTGTT1355650.032.8443373
CTCGTAT18300.029.45560645
GTATGCC19750.028.71396648
CTTGTGT72900.027.5487482
GTGTTTG1771650.025.2090455
CCTTGTG53050.025.1340771
CAGTCAC28150.021.50523228
TTGAGTC121400.021.3844834
TTTGAGT273850.020.9530533
ATGCCGT28000.020.88032250
GATTTTT177150.020.7969386
AGTCACC29700.020.61899229
GGTAACT72350.020.3641781
Produced by FastQC (version 0.11.5)