FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36842064
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501100.13601300947742775No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC498610.13533715157760978No Hit
CTTTGTGTTTGAGAGGTGTAGGATAAGTGGGAGCCTCCGGGCGCAAGTGA391620.10629697619547049No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC386820.1049941175934117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36200.044.38562447
TCGTATG36350.043.7185246
ACTTTGT1654350.042.5866241
TATGCCG39700.039.9477749
TTTGTGT3508500.038.7437252
CTCGTAT41150.037.68213745
CTTTGTG3676250.036.858891
GTATGCC43800.036.52540648
TGTGTTT3924200.036.2121434
CTCTAGG45550.035.03104434
TTGTGTT4061900.034.9251983
TCTAGGA48800.032.9144535
CTTGTGT182750.030.460252
GGTAACT209150.029.9538041
CCTTGTG117850.028.985251
CCGTCTT56200.028.15845153
AGTCACT58000.027.933429
ATGCCGT58050.027.74265950
GTGTTTG5309500.026.7586985
CAGTCAC62200.026.15937828
Produced by FastQC (version 0.11.5)