FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_11b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_11b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19346387
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC215980.11163841599984535No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC196560.10160036600115566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT774300.040.7786941
CGTATGC19700.039.09173247
TCGTATG20000.038.6784846
TTTGTGT1470200.036.4041252
CTTTGTG1557050.034.268341
TGTGTTT1672100.033.623674
TATGCCG22850.033.3994449
TTGTGTT1723050.032.5707133
ACACTAT27950.031.6764838
CTCGTAT24750.031.25493245
CACTATC27950.030.55178339
TATCTCG27000.029.29476542
GGTAACT75250.028.4632631
GTATGCC27800.028.20638348
ACTATCT30450.026.66444640
CTTGTGT93200.025.6404442
AACACTA35150.025.28760737
GTGTTTG2313750.024.2899445
CTATCTC32650.024.22481741
CCTTGTG67150.023.2970681
Produced by FastQC (version 0.11.5)