FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19323312
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271880.1407005175924293No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC264010.13662771682204375No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC216890.11224266316250547No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGTCTCATCTCGTA203300.105209707321395TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32000.050.65014347
TATGCCG32800.049.9524249
TCGTATG31900.049.59910646
GTATGCC35050.046.54383548
CTCGTAT32950.045.0433745
ACTTTGT796500.042.940321
CGTCTCA38600.042.88156537
GTCTCAT39950.040.20616538
GTCACAT43550.039.53140630
CCGTCTT39300.039.4655553
ATGCCGT40900.039.37547350
TTTGTGT1750650.039.3042342
CTTTGTG1819650.037.6764371
AGTCACA46350.036.91711829
TGTGTTT1989450.036.2848434
CAGTCAC47400.035.95057328
TTGTGTT2064250.034.900993
ACATCGT50200.034.3652733
CATCTCG43700.032.7566442
TCACATC52950.032.71231531
Produced by FastQC (version 0.11.5)