FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_10b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_10b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22302218
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGG106100.056.1353536
AGTCACT111800.054.05404729
CACTACA112950.053.6900632
ACTACAT112850.053.61384233
CAGTCAC112900.053.2160128
TACATCG116450.051.65682235
CTACATC116800.051.56090534
CCAGTCA123500.048.3357327
TCCAGTC126150.047.4867826
ACTCCAG130100.045.93620724
GTCTGAA136100.044.57943718
ACGTCTG136750.044.26459516
CTGAACT139550.043.45274420
TGAACTC140100.042.98110221
CGCGTAT12100.042.5236445
AACTCCA142150.042.287623
CACGTCT146200.041.52282715
GAACTCC148600.040.5933822
ACACGTC150250.040.35700214
CTCCAGT150650.039.9262125