FastQCFastQC Report
Wed 28 Nov 2018
HG7FTBGX9_n01_Maizes_10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FTBGX9_n01_Maizes_10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12328321
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCTAAATCTCGTA268500.21779121422941536TruSeq Adapter, Index 4 (97% over 38bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158490.128557651930056No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150160.12180085187593671No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC146950.11919709099073587No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC141820.11503594041719063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG31150.063.48584446
TATGCCG31950.062.45384649
CCTAAAT32400.061.34818338
CGTATGC32850.060.73637447
CTCGTAT31300.060.72093245
GTATGCC32800.060.61798548
GACCTAA35900.056.6341436
ATGCCGT36950.054.0031650
CCGTCTT36850.053.2975253
AGTCACC39500.051.38228629
CAGTCAC40150.050.46205528
GTCACCG40800.049.65873330
ACCTAAA40700.049.52542537
GCTTGAA40250.049.1570562
TGCCGTC40800.048.73586751
CCGACCT42050.048.68382634
TGCTTGA42900.045.7118461
TCCAGTC45150.045.18361726
CCAGTCA45500.044.68205627
TAAATCT44750.042.1522340
Produced by FastQC (version 0.11.5)