FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46367346
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27896406.016389206317739No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAA465790.10045647210431237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC52600.029.652731
CGATCCG53500.029.0571123
TCGATCC53750.028.7775362
GATCCGA57800.026.7108884
ATCCGAC58250.026.5324675
CGACTCG58200.026.4220858
ACTCGGT59150.026.10249710
GACTCGG61500.025.079969
TCGGTGC63000.024.50501812
CCGACTC64000.024.3183697
CTCGGTG65750.023.67019511
CCGAGTA30650.023.4632992
AGTAGCT97900.023.4029245
AGGCTGA114400.023.15687211
GTAGCTG99250.023.1160096
TACAGGC114500.023.0939917
CCCGAGT31700.023.0852811
CGGTGCC67950.022.83334713
GATTACA129700.022.80046314
TTACAGG123700.022.75450316
Produced by FastQC (version 0.11.5)