FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC9_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC9_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55355043
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC166350.024.4015825
AGGCCGA277650.024.007411
CCGAGTA228950.023.9894942
CGAGTAG229600.023.9296253
TTGGGAG965200.023.8564136
GTAGCTG797250.023.7021796
GGGATTA1077550.023.63938312
AGTAGCT808050.023.6096045
AGGCTGA991700.023.48597111
GGATTAC1020200.023.46922113
ATCGCTT200800.023.39543227
GATTACA1043650.023.32857314
CCCGAGT242500.023.2913511
TTTGGGA773850.023.1706775
TGGGATT1129200.023.14520611
CGCTTGA196350.023.14529
ATTACAG1051150.023.07950615
TCGCTTG198100.023.07317528
TACAGGC948750.022.97524317
CGTGAGC235900.022.85080123