FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC8_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC8_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27173121
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC72250.024.66985525
CGAGTAG112250.024.4249063
CCGAGTA112950.024.3136712
AGGCCGA130950.023.7700211
GGATTAC482100.023.73120713
AGTAGCT389800.023.7131655
TTGGGAG451250.023.7063886
GGGATTA514550.023.69920312
CCCGAGT118800.023.6683651
GTAGCTG383050.023.6456366
GATTACA496700.023.46357214
AGGCTGA460050.023.41133111
ATCGCTT90100.023.34339727
TGGGATT541450.023.19844211
CGTGAGC111800.023.09607723
ATTACAG503300.023.06336815
TTTGGGA360600.022.9853465
ACTTTGG332450.022.862073
TACAGGC457300.022.85532217
CGCTTGA88350.022.82416729
Produced by FastQC (version 0.11.5)