FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC7_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC7_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25296131
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275460.10889412297872747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGA124050.024.71714811
GAATCGC73200.024.6035525
CGAGTAG103350.024.4140973
CCGAGTA103900.024.267832
GGGATTA489600.023.90931512
GGATTAC462750.023.90285513
CCCGAGT107500.023.876751
ATCGCTT90150.023.76048727
GTAGCTG362900.023.749816
TTGGGAG439200.023.7215926
GATTACA478200.023.69045814
AGGCTGA443800.023.66763911
AGTAGCT369650.023.6639565
CGTGAGC106050.023.39815923
AATCGCT79450.023.33144826
CGCTTGA88100.023.31145329
ATTACAG483150.023.30965615
TACAGGC423300.023.3034317
TGGGATT518800.023.24900211
TCGCTTG89900.023.1719228
Produced by FastQC (version 0.11.5)