FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC5_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC5_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27239267
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC96300.025.33263225
AGGCCGA165000.025.27181211
TTGGGAG560300.025.0213766
CCGAGTA136000.024.945672
CGAGTAG137500.024.9332853
GTAGCTG463450.024.7547636
AGTAGCT467000.024.6991315
ATCGCTT117250.024.57393627
GGGATTA621000.024.55378212
AATCGCT100950.024.51878226
AGGCTGA570400.024.47759211
GGATTAC589150.024.45490813
GATTACA600150.024.4082914
TTTGGGA447050.024.3380835
CGTGAGC139100.024.1897523
TGGGATT647700.024.1697711
CGCTTGA115600.024.14789629
TCGCTTG116850.024.07492328
ACTTTGG410500.024.0429063
ATTACAG606200.023.97786515
Produced by FastQC (version 0.11.5)