FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC4_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC4_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29325922
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325790.1110928413435731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG143650.025.3762263
CCGAGTA144900.025.2621632
AGGCCGA165200.025.1571911
GAATCGC94100.024.98641225
TTGGGAG577400.024.7909766
GTAGCTG495800.024.753396
AGTAGCT499800.024.716445
GGGATTA652550.024.64033912
CCCGAGT149950.024.5280021
AGGCTGA578300.024.50817111
GGATTAC617650.024.49831213
GATTACA630000.024.4031814
CGTGAGC141000.024.19357123
TGGGATT677750.024.16207511
ATTACAG633450.024.05992315
ATCGCTT115900.024.04486727
CGCTTGA112650.023.9689729
TTTGGGA466450.023.9611975
TACAGGC559700.023.94652717
AATCGCT100700.023.73347726
Produced by FastQC (version 0.11.5)