FastQCFastQC Report
Thu 22 Nov 2018
HG7FNBGX9_n02_ATAC3_11-14-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG7FNBGX9_n02_ATAC3_11-14-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30707271
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG154050.025.3538133
GAATCGC106650.025.18488925
CCGAGTA155500.024.9557252
AGGCCGA185150.024.70699711
TTGGGAG625850.024.6849466
GGGATTA691950.024.54928612
GGATTAC655650.024.45600313
GTAGCTG536550.024.4538366
ATCGCTT129250.024.43901627
AGGCTGA642250.024.35833511
AGTAGCT543050.024.3236895
AATCGCT113150.024.24493426
GATTACA672800.024.21424714
TTTGGGA498900.024.1644735
CCCGAGT163450.024.0676541
CGCTTGA125950.023.97298429
CGTGAGC152200.023.95095423
TGGGATT727750.023.90781611
TCGCTTG128400.023.84090828
ATTACAG675300.023.83078415